Chinese Tuina Downregulates the Elevated Levels of Tissue Plasminogen Activator in Sciatic Nerve Injured Sprague-Dawley Rats

• Published in: Chinese journal of integrative medicine Vol. 23; no. 8; pp. 617 - 624
• Main Authors: Pan, Fan, Yu, Tian-yuan, Wong, Steven, Xian, Si-tong, Lu, Meng-qian, Wu, Jian-cong, Gao, Yu-feng, Li, Xiao-qin, Geng, Nan, Yao, Bin-bin
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.08.2017
• Subjects: Acupuncture Research; Animals; Down-Regulation; Male; Medicine; Medicine & Public Health; Medicine, Chinese Traditional; Muscles - pathology; Nerve Crush; Organ Size; PAI-1; Plasminogen Activator Inhibitor 1 - metabolism; Rats, Sprague-Dawley; Sciatic Nerve - injuries; Sciatic Nerve - pathology; SD大鼠; t-PA; Tissue Plasminogen Activator - metabolism; 周围神经损伤; 坐骨神经; 水; 组织型纤溶酶原激活剂; 组织纤溶酶原激活物; Chinese medicine; physical therapy; massage; peripheral neuropathy
• ISSN: 1672-0415; 1993-0402
• DOI: 10.1007/s11655-015-2142-1

Objective：To elucidate the mechanism of Chinese tuina in treating sciatic nerve crush injury,and to detect the levels of tissue plasminogen activator（t PA）and plasminogen activator inhibitor-1（PAI-1）,which is thought to play an important role in nerve regeneration.Methods：Thirty-two adult male Sprague-Dawley rats were subjected to sciatic nerve crush injury and 16 rats（sham-operated group）went through a sham operation.Control group was given no treatment while tuina group received tuina therapy since day 7 post-surgery.Tuina treatment was performed once a day and lasted for 20 days.The sciatic functional index was examined every 5days during the treatment session.The rats＇gastrocnemius muscles were evaluated for changes in mass and immunohistochemistry techniques were performed to detect the levels of tP A and PAI-1.Results：Tuina therapy improved the motor function of sciatic nerve injured rats（P〈0.05）,however,it did not increase muscle volume（P〈0.05）.Tuina downregulated the levels of t PA and PAI-1（P〈0.05）.Conclusions：The present study implies that tuina treatment could accelerate rehabilitation of peripheral nerve injury.