The membrane-bound sorbosone dehydrogenase of Gluconacetobacter liquefaciens is a pyrroloquinoline quinone-dependent enzyme

• Published in: Enzyme and microbial technology Vol. 137; p. 109511
• Main Authors: Yakushi, Toshiharu, Takahashi, Ryota, Matsutani, Minenosuke, Kataoka, Naoya, Hours, Roque A., Ano, Yoshitaka, Adachi, Osao, Matsushita, Kazunobu
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.06.2020
• Subjects: 2-Keto-l-gulonic acid; Acetic acid bacteria; Gluconacetobacter; l-Sorbosone; Pyrroloquinoline quinone; Sorbosone dehydrogenase; Ga. liquefacuens; 2KGLA; Q1; Sorbosone dehydrogenase; MOPS; Q2; 2-Keto-l-gulonic acid; DCPIP; CcPDH; Kg. vulgare; Gluconacetobacter; sGDH; A. calcoaceticus; PQQ; Q; Pyrroloquinoline quinone; HEPES; PMS; G. oxydans; SNDH; MES; Acetic acid bacteria; E. coli; l-Sorbosone
• ISSN: 0141-0229; 1879-0909
• DOI: 10.1016/j.enzmictec.2020.109511

[Display omitted] •Sorbosone dehydrogenase (SNDH) of Gluconacetobacter liquefaciens was characterized.•The SNDH activity is dependent on pyrroloquinoline quinone (PQQ).•Natural electron acceptor for SNDH is most likely membranous ubiquinone.•A structure was modeled with soluble PQQ-dependent pyranose dehydrogenase in silico. Membrane-bound sorbosone dehydrogenase (SNDH) of Gluconacetobacter liquefaciens oxidizes l-sorbosone to 2-keto-l-gulonic acid (2KGLA), a key intermediate in vitamin C production. We constructed recombinant Escherichia coli and Gluconobacter strains harboring plasmids carrying the sndh gene from Ga. liquefaciens strain RCTMR10 to identify the prosthetic group of SNDH. The membranes of the recombinant E. coli showed l-sorbosone oxidation activity, only after the holo-enzyme formation with pyrroloquinoline quinone (PQQ), indicating that SNDH is a PQQ-dependent enzyme. The sorbosone-oxidizing respiratory chain was thus heterologously reconstituted in the E. coli membranes. The membranes that contained SNDH showed the activity of sorbosone:ubiquinone analogue oxidoreductase. These results suggest that the natural electron acceptor for SNDH is membranous ubiquinone, and it functions as the primary dehydrogenase in the sorbosone oxidation respiratory chain in Ga. liquefaciens. A biotransformation experiment showed l-sorbosone oxidation to 2KGLA in a nearly quantitative manner. Phylogenetic analysis for prokaryotic SNDH homologues revealed that they are found only in the Proteobacteria phylum and those of the Acetobacteraceae family are clustered in a group where all members possess a transmembrane segment. A three-dimensional structure model of the SNDH constructed with an in silico fold recognition method was similar to the crystal structure of the PQQ-dependent pyranose dehydrogenase from Coprinopsis cinerea. The structural similarity suggests a reaction mechanism under which PQQ participates in l-sorbosone oxidation.