Spontaneous Induction of Endogenous Murine Leukemia Virus-Related Antigen Expression during Short-Term in Vitro Incubation of Mouse Lymphocytes

Short-term lymphocyte cultures from mouse thymus, spleen, or lymph nodes were studied for the presence of murine leukemia virus group-specific antigens with an immunofluorescence test using rat immune sera against syngeneic cells infected with the radiation leukemia virus or its pseudotype of murine...

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Published inProceedings of the National Academy of Sciences - PNAS Vol. 71; no. 5; pp. 2008 - 2012
Main Authors Lonai, Peter, Decleve, Alain, Kaplan, Henry S.
Format Journal Article
LanguageEnglish
Published United States National Academy of Sciences of the United States of America 01.05.1974
National Acad Sciences
Subjects
Animals
Antigens
Antigens, Viral - analysis
Antiserum
B lymphocytes
Biological Sciences: Immunology
Cell lines
Cells, Cultured
Cultured cells
Cycloheximide - pharmacology
Cytarabine - pharmacology
Dactinomycin - pharmacology
Fluorescent Antibody Technique
Hybridization, Genetic
Leukemia
Leukemia Virus, Murine - immunology
Lymph Nodes - immunology
Lymphocytes
Lymphocytes - immunology
Mice
Mice, Inbred Strains
Mitomycins - pharmacology
Murine leukemia virus
Oligonucleotides - pharmacology
Spleen - immunology
Thymocytes
Thymus Gland - immunology
Time Factors
Viruses
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Summary:Short-term lymphocyte cultures from mouse thymus, spleen, or lymph nodes were studied for the presence of murine leukemia virus group-specific antigens with an immunofluorescence test using rat immune sera against syngeneic cells infected with the radiation leukemia virus or its pseudotype of murine sarcoma virus and goat and rabbit antisera against purified murine leukemia virus group-specific antigen. Antigens reacting with these sera appeared in the cultured lymphocytes within 24 hr, and the proportion of immunofluorescent-positive cells increased to 25-80% by the second or third day of cultivation, concomitantly with a decrease in cell viability. The appearance of these antigens can be suppressed by inhibitors of DNA (mitomycin-C), RNA (actinomycin-D, cordycepin, and polyadenylic acid), and protein (cycloheximide) synthesis. No infectious virus could be detected by the immunofluorescence and XC-cell tests. The observed phenomenon appears to represent the spontaneous partial derepression of endogenous murine leukemia virus replication in lymphocytes during short-term in vitro cultivation.
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ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.71.5.2008