Temporal dynamics of soil bacterial network regulate soil resistomes

• Published in: Environmental microbiology Vol. 25; no. 2; pp. 505 - 514
• Main Authors: Xiang, Qian, Zhu, Dong, Qiao, Min, Yang, Xiao‐Ru, Li, Gang, Chen, Qing‐Lin, Zhu, Yong‐Guan
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01.02.2023; Wiley Subscription Services, Inc
• Subjects: Anti-Bacterial Agents - pharmacology; Antibiotic resistance; Antibiotics; Bacteria; Bacteria - genetics; Disease resistance; Drug resistance; Drug Resistance, Microbial - genetics; Dynamics; Ecological effects; Environmental changes; Environmental management; Field investigations; Genes; Genes, Bacterial; Microorganisms; Networks; Seasonal variation; Seasonal variations; Seasons; Soil; Soil bacteria; Soil dynamics; Soil Microbiology; Soil microorganisms; Soil resistance; Soils; Summer; Topology; Watersheds
• ISSN: 1462-2912; 1462-2920
• DOI: 10.1111/1462-2920.16298

Soil bacteria are diverse and form complicated ecological networks through various microbial interactions, which play important roles in soil multi‐functionality. However, the seasonal effects on the bacterial network, especially the relationship between bacterial network topological features and soil resistomes remains underexplored, which impedes our ability to unveil the mechanisms of the temporal‐dynamics of antibiotic resistance genes (ARGs). Here, a field investigation was conducted across four seasons at the watershed scale. We observed significant seasonal variation in bacterial networks, with lower complexity and stability in autumn, and a wider bacterial community niche in summer. Similar to bacterial communities, the co‐occurrence networks among ARGs also shift with seasonal change, particularly with respect to the topological features of the node degree, which on average was higher in summer than in the other seasons. Furthermore, the nodes with higher betweenness, stress, degree, and closeness centrality in the bacterial network showed strong relationships with the 10 major classes of ARGs. These findings highlighted the changes in the topological properties of bacterial networks that could further alter antibiotic resistance in soil. Together, our results reveal the temporal dynamics of bacterial ecological networks at the watershed scale, and provide new insights into antibiotic resistance management under environmental changes.