Evidence for the Absence of the G-T-ψ -C Sequence from Two Mammalian Initiator Transfer RNAs

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 70; no. 4; pp. 1046 - 1049
• Main Author: Petrissant, Guy
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences of the United States of America 01.04.1973; National Acad Sciences
• Subjects: Animals; Base Sequence; Biological Sciences: Biochemistry; Chromatography; Chromatography, DEAE-Cellulose; Chromatography, Gel; Chromatography, Thin Layer; Cytosine Nucleotides - analysis; Escherichia coli - analysis; Guanine Nucleotides - analysis; Hydrolysis; Liver; Liver - analysis; Mammary glands; Mammary Glands, Animal - analysis; Nucleosides; Nucleosides - analysis; Nucleotides; Nucleotides - analysis; Oligonucleotides; Oligonucleotides - analysis; Phosphoric Diester Hydrolases; Phosphoric Monoester Hydrolases; Piperidines; Rabbits; Ribonucleases; RNA, Transfer - analysis; RNA, Transfer - isolation & purification; Sheep; Thymine Nucleotides - analysis; Transfer RNA; Yeasts
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.70.4.1046

Analyses were performed on the purified initiator tRNA from rabbit liver to test for the presence of ψ p and Tp in this molecule. Neither of these nucleotides could be detected after hydrolysis by piperidine, NaOH, or T2 RNase. Similarly, digestion with venom phosphodiesterase plus phosphatase failed to release any pseudouridine or ribothymidine. Identical results were obtained with the initiator tRNA from sheep mammary gland. The absence of these nucleosides was confirmed by pancreatic and T1 RNase digestion of the rabbit-liver initiator tRNA. The classical G-T-ψ -C sequence was not detected in this molecule. An A-U-C-G sequence has been identified; it may possibly replace the G-T-ψ -C sequence.