1H–NMR Metabolomic Biomarkers of Poor Outcome after Hemorrhagic Shock are Absent in Hibernators

• Published in: PloS one Vol. 9; no. 9; p. e107493
• Main Authors: Bogren, Lori K., Murphy, Carl J., Johnston, Erin L., Sinha, Neeraj, Serkova, Natalie J., Drew, Kelly L.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 2014; Public Library of Science (PLoS)
• Subjects: Activation; Animals; Biochemistry; Bioindicators; Biology and Life Sciences; Biomarkers; Biomarkers - blood; Blood pressure; Body temperature; Cold; Computer and Information Sciences; Experiments; Glucose metabolism; Glycolysis; Ground squirrels; Hemorrhage; Hibernation; Homeostasis; Hypoxia; Injuries; Ischemia; Laboratory animals; Lipid metabolism; Lipids - blood; Magnetic Resonance Spectroscopy; Male; Medical research; Medical wastes; Medicine and Health Sciences; Metabolic rate; Metabolism; Metabolites; Metabolome; Metabolomics; NMR; Nuclear magnetic resonance; Polar environments; Rats; Rats, Sprague-Dawley; Reperfusion; Reperfusion Injury - blood; Reperfusion Injury - prevention & control; Rodents; Sciuridae; Seasons; Shock, Hemorrhagic - blood; Shock, Hemorrhagic - therapy; Squirrels; Temperature effects; Trauma; Treatment Outcome; Winter; United States > US; Alaska; Arctic region
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0107493

Hemorrhagic shock (HS) following trauma is a leading cause of death among persons under the age of 40. During HS the body undergoes systemic warm ischemia followed by reperfusion during medical intervention. Ischemia/reperfusion (I/R) results in a disruption of cellular metabolic processes that ultimately lead to tissue and organ dysfunction or failure. Resistance to I/R injury is a characteristic of hibernating mammals. The present study sought to identify circulating metabolites in the rat as biomarkers for metabolic alterations associated with poor outcome after HS. Arctic ground squirrels (AGS), a hibernating species that resists I/R injury independent of decreased body temperature (warm I/R), was used as a negative control. Male Sprague-Dawley rats and AGS were subject to HS by withdrawing blood to a mean arterial pressure (MAP) of 35 mmHg and maintaining the low MAP for 20 min before reperfusing with Ringers. The animals' temperature was maintained at 37 ± 0.5 °C for the duration of the experiment. Plasma samples were taken immediately before hemorrhage and three hours after reperfusion. Hydrophilic and lipid metabolites from plasma were then analyzed via 1H-NMR from unprocessed plasma and lipid extracts, respectively. Rats, susceptible to I/R injury, had a qualitative shift in their hydrophilic metabolic fingerprint including differential activation of glucose and anaerobic metabolism and had alterations in several metabolites during I/R indicative of metabolic adjustments and organ damage. In contrast, I/R injury resistant AGS, regardless of season or body temperature, maintained a stable metabolic homeostasis revealed by a qualitative 1H-NMR metabolic profile with few changes in quantified metabolites during HS-induced global I/R. An increase in circulating metabolites indicative of anaerobic metabolism and activation of glycolytic pathways is associated with poor prognosis after HS in rats. These same biomarkers are absent in AGS after HS with warm I/R.