Apogamous sporophyte development through spore reproduction of a South Asia's critically endangered fern: Pteris tripartita Sw

• Published in: Asian Pacific Journal of Reproduction Vol. 4; no. 2; pp. 135 - 139
• Main Authors: Ravi, Baskaran Xavier, Varuvel, Geo Vigila Antony, kilimas, Rajan, Robert, Jeyachandran
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.06.2015; Wolters Kluwer Medknow Publications
• Subjects: BAP; Gametophytes; Pteris tripartita; Spore; Sporophytes; Gametophytes; Pteris tripartita; BAP; Spore; Sporophytes
• ISSN: 2305-0500
• DOI: 10.1016/S2305-0500(15)30010-5

To develop an efficient reproducible protocol for a critically endangered fern, Pteris tripartita (P. tripartita) Sw. through spore culture. The spore derived gametophytes were grown in a half strength MS medium which sub-cultured after three months to MS medium containing growth regulators like BAP, Kinetin and GA3 with 3% sucrose. The highest mean number of sporophytes (64.40) induced with 4.16 cm of length and also formed 1.51 cm of root length at 4 mg/L of BAP. On MS medium supplemented with KIN, 56.03 mean numbers of sporophytes with 2.61 cm along with 1.39 cm length of root were obtained in 4 mg/L of KIN. And, the highest mean numbers of juvenile sporophytes (59.66) were induced with 2.64 cm mean lengths of sporophytes with 2.23 cm of root length in 4 mg/L of GA3 in the MS culture medium. The in vitro developed plantlets were washed with distilled water and grown in small cups containing sterilized soil, sand and vermiculite (1:1:1). The acclimatized plantlet showed 90% of a survival rate. In this study, reproducible protocol could be useful for their rehabilitation and also to reduce the pressure on natural population in Asian habitat.