Sustained perfusion of revascularized bioengineered livers heterotopically transplanted into immunosuppressed pigs
Implanted bioengineered livers have not exceeded three days of continuous perfusion. Here we show that decellularized whole porcine livers revascularized with human umbilical vein endothelial cells and implanted heterotopically into immunosuppressed pigs whose spleens had been removed can sustain pe...
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Published in | Nature biomedical engineering Vol. 4; no. 4; pp. 437 - 445 |
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Main Authors | , , , , , , , , , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
England
Nature Publishing Group
01.04.2020
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Subjects | |
Online Access | Get full text |
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Summary: | Implanted bioengineered livers have not exceeded three days of continuous perfusion. Here we show that decellularized whole porcine livers revascularized with human umbilical vein endothelial cells and implanted heterotopically into immunosuppressed pigs whose spleens had been removed can sustain perfusion for up to 15 days. We identified peak glucose consumption rate as a main predictor of the patency of the revascularized bioengineered livers (rBELs). Heterotopic implantation of rBELs into pigs in the absence of anticoagulation therapy led to sustained perfusion for three days, followed by a pronounced immune responses directed against the human endothelial cells. A 10 day steroid-based immunosuppression protocol and a splenectomy at the time of rBEL implantation reduced the immune responses and resulted in continuous perfusion of the rBELs for over two weeks. We also show that the human endothelial cells in the perfused rBELs colonize the liver sinusoids and express sinusoidal endothelial markers similar to those in normal liver tissue. Revascularized liver scaffolds that can maintain blood perfusion at physiological pressures might eventually help to overcome the chronic shortage of transplantable human livers. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 MFS, JJR, DSD, SLN designed the study; AY & VZ performed the HUVEC and rBEL culture; MFS, DJ, HSC, YL, BA, EN, TM and SLN performed the surgical procedures; GM performed the CT; BA, RCH, ML performed electron microscopy; AJVW & CRP performed the RNA-seq analysis, MFS, DJ, JJR, BDA, DSD, RCH, VHS, ML, SLN analyzed the experimental data; BDA drafted the figures; MFS, JJR, BDA, SLN wrote the manuscript. RCH, VHS, ML reviewed and edited the manuscript. Author Contributions |
ISSN: | 2157-846X 2157-846X |
DOI: | 10.1038/s41551-019-0460-x |