In Vitro Attenuation of Thermal-Induced Protein Denaturation by Aerial Parts of Artemisia scoparia

• Published in: Journal of evidence-based complementary & alternative medicine Vol. 20; no. 1; pp. 9 - 12
• Main Authors: Khan, Murad Ali, Khan, Haroon, Tariq, Shafiq Ahmad, Pervez, Samreen
• Format: Journal Article
• Language: English
• Published: Los Angeles, CA SAGE Publications 01.01.2015
• Subjects: Albumins - chemistry; Analysis of Variance; Artemisia; Artemisia - chemistry; Aspirin - pharmacology; Flavonoids - analysis; Flavonoids - chemistry; Plant Components, Aerial - chemistry; Plant Extracts - chemistry; Plant Extracts - pharmacology; Protein Denaturation - drug effects; Scoparia; flavonoid contents; extract and aqueous fraction; protein denaturation potential; Artemisia scoparia
• ISSN: 2156-5872; 2156-5899
• DOI: 10.1177/2156587214548458

The goal of this study was to explore the aerial parts of Artemisia scoparia (crude extract, total flavonoid contents, and aqueous fraction) for protein denaturation potential. The crude extract provoked marked attenuation of thermal-induced denatured protein in a concentration-dependent manner with maximum inhibition of 54.05 μg/mL at 500 μg/mL and IC50 of 449.66 μg/mL. When total flavonoid contents were studied, it illustrated most dominant activity concentration dependently with maximum amelioration of 62.16 μg/mL at 500 μg/mL and IC50 of 378.35 μg/mL. The aqueous fraction also exhibited significant activity with maximum of 56.75% inhibition at 500 μg/mL and IC50 of 445.10 μg/mL. It can be concluded on the basis of the results that the crude extract, flavonoid contents, and aqueous fraction of the plant possessed significant inhibition on thermal-induced denatured protein.