Systematic status of the Glycine tomentella and G. tabacina species complexes (Fabaceae) based on ITS sequences of nuclear ribosomal DNA

• Published in: Journal of plant research Vol. 114; no. 1116; pp. 435 - 442
• Main Authors: Hsing, Y.I.C. (Academia Sinica, Taipei (Taiwan)), Hsieh, J.S, Peng, C.I, Chou, C.H, Chiang, T.Y
• Format: Journal Article
• Language: English
• Published: Tokyo Springer Nature B.V 01.12.2001
• Subjects: DNA; Genetic diversity; GLYCINE (GENUS); Glycine dolichocarpa; Glycine tabacina; Glycine tomentella; NUCLEOTIDE SEQUENCE; NUCLEUS; PHYLOGENY; Plant biology; Plant populations; Population genetics; Ribosomal DNA; RIBOSOMES; SPECIES
• ISSN: 0918-9440; 1618-0860
• DOI: 10.1007/pl00014009

The phylogeny of the subgenus Glycine was reconstructed baaed on nucleotide sequences of the Internal transcribed spacer (ITS) region of the nuclear ribosomal DNA to examine the systematic status of the G. tomentella and G. tabacina species complexes. Rooted at the subgenus Soja (2 species, 7 accessions), parsimony analysis was conducted for 17 species (31 accessions) of the subgenus Glycine, including 9 and 6 populations of G. tomentella s.l. and G. tabacina s.l., respectively. The nrITS phylogeny indicated polyphyly of G. tomentella s.l. as well as G. tabacina s.l. In the G. tomentella species complex, larger legumes, narrower leaflets, and deflexed indumentum hairs differentiated G. dolichocarpa from G. tomentella s.s. The tetraploid G. dolichocarpa (2n=80) and aneuploid G. tomentella (2n=38) represented Independent lineages from another clade of the remaining dlploid (2n=40) and tetraploid species with a DD genome type, Tetraploid G, tabacina (2n=80) was closely related to G. dolichocarpa instead of the diploid G. tabacina (2n=40) with a BB genome type. The nrITS phylogeny suggested allopolyploidy of G. dolichocarpa and of the tetraploid G. tabacina, both of which possibly share a common parental species with an AA genome type. Their phylogenetic affinity also indicated biased inheritance of the nrDNA ITS and a possible dominant role of the AA genome. Phylogenetically, G. dolichocarpa and alltotetraploid G. tabacina should be recognized as distinct species.