The use of peptides for immunodiagnosis of human Chagas disease

• Published in: Amino acids Vol. 56; no. 1; p. 35
• Main Authors: Ribeiro, Anna Julia, Silva, Kamila Alves, Lopes, Lucas da Silva, Resende, Carlos Ananias Aparecido, Couto, Carolina Alves Petit, Gandra, Isadora Braga, Pereira, Isabela Amorim Gonçalves, Barcelos, Isabelle Caroline Dos Santos, Pereira, Sabrina Paula, Xavier, Sandra Rodrigues, Tavares, Grasiele de Sousa Viera, Machado, Juliana Martins, Da Paz, Mariana Campos, Chávez-Fumagalli, Miguel Angel, Coelho, Eduardo Antonio Ferraz, Giunchetti, Rodolfo Cordeiro, Chaves, Ana Thereza, Dutra, Walderez Ornelas, Gonçalves, Ana Alice Maia, Galdino, Alexsandro Sobreira
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 02.05.2024; Springer Nature B.V
• Subjects: Analytical Chemistry; Antigens; Antigens, Protozoan - blood; Antigens, Protozoan - immunology; Biochemical Engineering; Biochemistry; Biomedical and Life Sciences; Chagas disease; Chagas Disease - blood; Chagas Disease - diagnosis; Chagas Disease - immunology; Enzyme-linked immunosorbent assay; Enzyme-Linked Immunosorbent Assay - methods; Epitopes; Humans; Immunodiagnosis; Immunogenicity; Immunologic Tests - methods; Life Sciences; Neurobiology; Peptides; Peptides - chemistry; Peptides - immunology; Proteomics; Protozoa; Public health; Review Article; Serologic Tests - methods; Serology; Trypanosoma cruzi; Trypanosoma cruzi - immunology; Chagas disease; Imunodiagnosis; peptide; Serodiagnosis; Trypanosoma cruzi
• ISSN: 1438-2199; 0939-4451; 1438-2199
• DOI: 10.1007/s00726-024-03394-6

Chagas disease, caused by the protozoa Trypanosoma cruzi , continues to be a serious public health problem in Latin America, worsened by the limitations in its detection. Given the importance of developing new diagnostic methods for this disease, the present review aimed to verify the number of publications dedicated to research on peptides that demonstrate their usefulness in serodiagnosis. To this end, a bibliographic survey was conducted on the PubMed platform using the keyword “peptide” or “epitope” combined with “Chagas disease” or “ Trypanosoma cruzi ”; “diagno*” or “serodiagnosis” or “immunodiagnosis”, without period restriction. An increasing number of publications on studies employing peptides in ELISA and rapid tests assays was verified, which confirms the expansion of research in this field. It is possible to observe that many of the peptides tested so far originate from proteins widely used in the diagnosis of Chagas, and many of them are part of commercial tests developed. In this sense, as expected, promising results were obtained for several peptides when tested in ELISA, as many of them exhibited sensitivity and specificity values above 90%. Furthermore, some peptides have been tested in several studies, confirming their diagnostic potential. Despite the promising results observed, it is possible to emphasize the need for extensive testing of peptides, using different serological panels, in order to confirm their potential. The importance of producing an effective assay capable of detecting the clinical stages of the disease, as well as new immunogenic antigens that enable new serological diagnostic tools for Chagas disease, is evident.