Banana Lectin from Musa paradisiaca Is Mitogenic for Cow and Pig PBMC via IL-2 Pathway and ELF1

The aim of the study was to gain deeper insights in the potential of polyclonal stimulation of PBMC with banana lectin (BanLec) from Musa paradisiaca. BanLec induced a marked proliferative response in cow and pig PBMC, but was strongest in pigs, where it induced an even higher proliferation rate tha...

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Bibliographic Details
Published inImmuno Vol. 1; no. 3; pp. 264 - 276
Main Authors Degroote, Roxane L., Korbonits, Lucia, Stetter, Franziska, Kleinwort, Kristina J. H., Schilloks, Marie-Christin, Amann, Barbara, Hirmer, Sieglinde, Hauck, Stefanie M., Deeg, Cornelia A.
Format Journal Article
LanguageEnglish
Published Sapporo MDPI AG 01.09.2021
Subjects
banana lectin
Biomedical research
Chromatography
complement pathway
Cytokines
Hogs
Immune system
Lectins
Lymphocytes
Musa paradisiaca
PBMC
Peptides
plant lectin
polyclonal cell stimulation
United States > US
Germany
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Summary:The aim of the study was to gain deeper insights in the potential of polyclonal stimulation of PBMC with banana lectin (BanLec) from Musa paradisiaca. BanLec induced a marked proliferative response in cow and pig PBMC, but was strongest in pigs, where it induced an even higher proliferation rate than Concanavalin A. Molecular processes associated with respective responses in porcine PBMC were examined with differential proteome analyses. Discovery proteomic experiments was applied to BanLec stimulated PBMC and cellular and secretome responses were analyzed with label free LC-MS/MS. In PBMC, 3955 proteins were identified. After polyclonal stimulation with BanLec, 459 proteins showed significantly changed abundance in PBMC. In respective PBMC secretomes, 2867 proteins were identified with 231 differentially expressed candidates as reaction to BanLec stimulation. The transcription factor “E74 like ETS transcription factor 1 (ELF1)” was solely enriched in BanLec stimulated PBMC. BanLec induced secretion of several immune regulators, amongst them positive regulators of activated T cell proliferation and Jak-STAT signaling pathway. Top changed immune proteins were CD226, CD27, IFNG, IL18, IL2, CXCL10, LAT, ICOS, IL2RA, LAG3, and CD300C. BanLec stimulates PBMC of cows and pigs polyclonally and induces IL2 pathway and further proinflammatory cytokines. Proteomics data are available via ProteomeXchange with identifier PXD027505.
ISSN:2673-5601
2673-5601
DOI:10.3390/immuno1030018