Mononuclear copper(II) nitrato complexes with methyl-substituted 4-nitropyridine N-oxide. Physicochemical and cytotoxic characteristics

• Published in: Journal of inorganic biochemistry Vol. 104; no. 2; pp. 153 - 160
• Main Authors: Puszko, Aniela, Krojcer, Anna, Pełczynska, Marzena, Wietrzyk, Joanna, Cieślak-Golonka, Maria, Jezierska, Julia, Adach, Anna, Kubiak, Maria
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.02.2010
• Subjects: Cell Line, Tumor; Cell Proliferation - drug effects; Cell Survival - drug effects; Chemical Phenomena; Coordination Complexes - chemistry; Coordination Complexes - pharmacology; Copper - chemistry; Copper complexes; Crystal structure; Cytotoxicity; Dose-Response Relationship, Drug; Electron Spin Resonance Spectroscopy; EPR spectra; Humans; Hydrogen Bonding; Infrared spectra; Ligands; Magnetic moments; Methyl-substituted 4-nitropyridine N-oxide; Models, Molecular; Molecular Structure; Oxides - chemistry; Pyridines - chemistry; Spectrophotometry, Infrared; Thermal decomposition; Thermodynamics; Thermal decomposition; Magnetic moments; Cytotoxicity; Infrared spectra; EPR spectra; Methyl-substituted 4-nitropyridine N-oxide; Copper complexes; Crystal structure
• ISSN: 0162-0134; 1873-3344
• DOI: 10.1016/j.jinorgbio.2009.10.016

Three new complexes, products of the interaction of Cu(NO 3) 2 and methyl-substituted 4-nitropyridine N-oxides were synthesized and characterized by elemental analysis, magnetic, spectroscopic (IR, FIR and EPR), thermal and X-ray methods. The complexes (magnetic moments 1.70–1.81 BM at 300 K) of general formula [Cu(H 2O)(NO 3) 2L 2], L = 2-methyl-4-nitropyridine N-oxide and [Cu(NO 3) 2 L′ 2], where L′ = 2,6-dimethyl- and 2,3,6-trimethyl-4-nitropyridine N-oxide were obtained. The compounds were unstable upon dissolution. The X-ray single crystal structure of Cu(II) complex with 2,6-dimethyl-4-nitropyridine N-oxide was determined and analysed. The compounds and free ligands were tested in vitro on the cytotoxic activity against MCF-7 and SW-707 human cancer cell lines. The complexes with 4-nitropyridine N-oxide (a reference) and 2-methyl-4-nitropyridine N-oxide show a significant anti-proliferative activity against studied cell lines. A reciprocal relationship between the activity and the number of methyl groups was observed. Both ligands and complexes are cytotoxic active but to the different cell lines.