Liver snips. A simple, rapid and reproducible method for studying metabolism in small fragments of tissue, as applied to glucuronidation in rat liver

A simple, rapid method is described of preparing intact cells as small (about 2mm) pieces of organized tissue capable of performing synthetic metabolic functions. It has been applied to the study of glucuronidation in rat liver. In this process, snips appear less damaged, more versatile and more act...

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Bibliographic Details
Published inBiochemical journal Vol. 202; no. 2; pp. 469 - 473
Main Authors Pollard, M R, Dutton, G J
Format Journal Article
LanguageEnglish
Published England 15.02.1982
Subjects
Aminophenols - metabolism
Animals
Cytological Techniques
Gases
Glucuronates - metabolism
Glucuronic Acid
In Vitro Techniques
Kinetics
Liver - cytology
Liver - metabolism
Male
Naphthols - metabolism
Rats
Rats, Inbred Strains
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Summary:A simple, rapid method is described of preparing intact cells as small (about 2mm) pieces of organized tissue capable of performing synthetic metabolic functions. It has been applied to the study of glucuronidation in rat liver. In this process, snips appear less damaged, more versatile and more active than tissue slices and yield results of reproducibility comparable with those with homogenates. From a comparison with the literature, snips glucuronidate the substrates employed at a rate much the same as in perfused preparations and some 30% less than the rate in isolated-hepatocyte suspensions; the advantages they offer in certain situations over these two techniques are discussed.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0264-6021
0306-3283
1470-8728
DOI:10.1042/bj2020469