Chiral separations using heparin and dextran sulfate in capillary zone electrophoresis

The utility of heparin and dextran sulfate as chiral mobile phase additives for the enantioresolution of chloroquine and chlorpheniramine in capillary zone electrophoresis was explored. Factors shown to affect the resolution of these analytes include pH, concentration of the buffer, concentration of...

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Bibliographic Details
Published inAnalytica chimica acta Vol. 307; no. 2; pp. 185 - 191
Main Authors Agyei, Nana M., Gahm, Kyung H., Stalcup, Apryll M.
Format Journal Article
LanguageEnglish
Published Elsevier B.V 30.05.1995
Subjects
Capillary zone electrophoresis
Dextrans
Electrophoresis
Enantiomer separation
Heparin
Electrophoresis
Dextrans
Heparin
Capillary zone electrophoresis
Enantiomer separation
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Summary:The utility of heparin and dextran sulfate as chiral mobile phase additives for the enantioresolution of chloroquine and chlorpheniramine in capillary zone electrophoresis was explored. Factors shown to affect the resolution of these analytes include pH, concentration of the buffer, concentration of the chiral additive and the molecular weight of the additive. In the presence of 2% heparin, optimized baseline resolution for chloroquine required 10 mM phosphate buffer, pH 6.0 while chlorpheniramine required pH 5.0. Similar conditions were required when using dextran sulfate although the best resolution for chlorpheniramine required 50 mM phosphate buffer.
ISSN:0003-2670
1873-4324
DOI:10.1016/0003-2670(95)00080-J