Ultrasensitive and specific fluorescence detection of a cancer biomarker via nanomolar binding to a guanidinium-modified calixarene

We designed a water-soluble guanidinium-modified calix[5]arene to target lysophosphatidic acid (LPA), an ideal biomarker for early diagnosis of ovarian and other gynecologic cancers, achieving binding on the nanomolar level. An indicator displacement assay, coupled with differential sensing, enabled...

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Published inChemical science (Cambridge) Vol. 9; no. 8; pp. 2087 - 2091
Main Authors Zheng, Zhe, Geng, Wen-Chao, Gao, Jie, Wang, Yu-Ying, Sun, Hongwei, Guo, Dong-Sheng
Format Journal Article
LanguageEnglish
Published CAMBRIDGE Royal Soc Chemistry 28.02.2018
Royal Society of Chemistry
Subjects
Binding
Biomarkers
Calixarenes
Chemistry
Chemistry, Multidisciplinary
Fluorescence
Physical Sciences
Science & Technology
INHIBITION
LYSOPHOSPHATIDIC ACID
ACETYLCHOLINE
SELECTIVE RECOGNITION
FLUOROGENIC PROBES
OVARIAN-CANCER
RECEPTORS
COMPLEXATION
MOLECULES
WATER
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Summary:We designed a water-soluble guanidinium-modified calix[5]arene to target lysophosphatidic acid (LPA), an ideal biomarker for early diagnosis of ovarian and other gynecologic cancers, achieving binding on the nanomolar level. An indicator displacement assay, coupled with differential sensing, enabled ultrasensitive and specific detection of LPA. Moreover, we show that using a calibration line, the LPA concentration in untreated serum can be quantified in the biologically relevant low μM range with a detection limit of 1.7 μM. The reported approach is feasible for diagnosing ovarian and other gynecologic cancers, particularly at their early stages.
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ISSN:2041-6520
2041-6539
DOI:10.1039/C7SC04989G