Effect of Storage Time on the Immunoreactivity of IgG Physically Adsorbed or Chemically Bound to Latex Beads

• Published in: Journal of colloid and interface science Vol. 184; no. 1; pp. 331 - 334
• Main Authors: Ortega-Vinuesa, J.L., Bastos-González, D., Hidalgo-Álvarez, R.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.12.1996; Elsevier
• Subjects: antibodies; Biological and medical sciences; Biotechnology; covalent coupling; Fundamental and applied biological sciences. Psychology; Immobilization of enzymes and other molecules; Immobilization techniques; latex immunoassays; Methods. Procedures. Technologies; polymer colloids; covalent coupling; latex immunoassays; antibodies; polymer colloids; Ball; Immunoglobulins; Stability; Adsorption; IgG; Immobilization; Covalent bond; Antigen antibody reaction; Comparative study; Latex
• ISSN: 0021-9797; 1095-7103
• DOI: 10.1006/jcis.1996.0627

The main purpose of the present work is to check whether the immunoreactivity of IgG molecules, chemically bound or adsorbed on latex, shows alteration after 6 months of storage. For this purpose, agglutination tests of sensitized carboxylated beads, measuring the immunoreactivity by turbidimetric monitoring after 5 min of immunoaggregation reaction with human C-reactive protein in a stopped-flow spectrophotometer, were repeated. The results show that while the covalently bound IgG maintains and in some cases improves on its previous immunological response, the passively adsorbed IgG shows loss of activity due to a decreased amount of adsorbed protein.