Elutability of Proteins from Aluminum-Containing Vaccine Adjuvants by Treatment with Surfactants

• Published in: Journal of colloid and interface science Vol. 197; no. 1; pp. 48 - 56
• Main Authors: Rinella, Joseph V., Workman, Ryan F., Hermodson, Mark A., White, Joe L., Hem, Stanley L.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.01.1998; Elsevier
• Subjects: aluminum hydroxide adjuvant; aluminum phosphate adjuvant; Applied microbiology; Biological and medical sciences; desorption of preadsorbed proteins; electrostatic repulsion; elutability; Fundamental and applied biological sciences. Psychology; micellar solubilization; Microbiology; solubilization; surfactant-induced elution; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); micellar solubilization; elutability; surfactant-induced elution; solubilization; aluminum phosphate adjuvant; desorption of preadsorbed proteins; aluminum hydroxide adjuvant; electrostatic repulsion; Elution; Adjuvant; Aluminium; Vaccine; Surfactant; Physicochemical properties; Protein
• ISSN: 0021-9797; 1095-7103
• DOI: 10.1006/jcis.1997.5230

The elutability of proteins from adjuvants in model vaccines composed of ovalbumin adsorbed by aluminum hydroxide adjuvant or lysozyme adsorbed by aluminum phosphate adjuvant following treatment with surfactant solutions was studied. Nonionic (Triton X-100, lauryl maltoside), zwitterionic (lauryl sulfobetaine), anionic (sodium dodecyl sulfate), and cationic (cetylpyridinium chloride, dodecyltrimethylammonium chloride) surfactants were investigated. Cetylpyridinium chloride produced the greatest degree of elution (60%) of ovalbumin from aluminum hydroxide adjuvant. Sodium dodecyl sulfate completely eluted lysozyme from aluminum phosphate adjuvant. The effectiveness of surfactants in removing preadsorbed proteins was directly related to their ability to denature the protein. Micellar solubilization and electrostatic repulsion may also contribute to desorption.