A modified exponential amplification reaction (EXPAR) with an improved signal-to-noise ratio for ultrasensitive detection of polynucleotide kinase

We applied a modified exponential amplification reaction (EXPAR) strategy to design a label-free and "one-pot" biosensor for ultrasensitive detection of polynucleotide kinase (PNK). This method was also successfully applied in the screening of PNK inhibitors and analysis of the endogenous...

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Published inChemical communications (Cambridge, England) Vol. 55; no. 53; pp. 7611 - 7614
Main Authors Zhang, Yu-Peng, Cui, Yun-Xi, Li, Xiao-Yu, Du, Yi-Chen, Tang, An-Na, Kong, De-Ming
Format Journal Article
LanguageEnglish
Published England Royal Society of Chemistry 07.07.2019
Subjects
Amplification
Biosensors
chemical compounds
chemical reactions
Design modifications
Kinases
screening
Signal to noise ratio
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Abstract We applied a modified exponential amplification reaction (EXPAR) strategy to design a label-free and "one-pot" biosensor for ultrasensitive detection of polynucleotide kinase (PNK). This method was also successfully applied in the screening of PNK inhibitors and analysis of the endogenous PNK activity at the single-cell level. We reported a modified exponential amplification reaction strategy and applied it to design an ultrasensitive biosensor for the detection of endogenous polynucleotide kinase activity at single-cell level.
AbstractList We applied a modified exponential amplification reaction (EXPAR) strategy to design a label-free and "one-pot" biosensor for ultrasensitive detection of polynucleotide kinase (PNK). This method was also successfully applied in the screening of PNK inhibitors and analysis of the endogenous PNK activity at the single-cell level.We applied a modified exponential amplification reaction (EXPAR) strategy to design a label-free and "one-pot" biosensor for ultrasensitive detection of polynucleotide kinase (PNK). This method was also successfully applied in the screening of PNK inhibitors and analysis of the endogenous PNK activity at the single-cell level.
We applied a modified exponential amplification reaction (EXPAR) strategy to design a label-free and "one-pot" biosensor for ultrasensitive detection of polynucleotide kinase (PNK). This method was also successfully applied in the screening of PNK inhibitors and analysis of the endogenous PNK activity at the single-cell level.
We applied a modified exponential amplification reaction (EXPAR) strategy to design a label-free and "one-pot" biosensor for ultrasensitive detection of polynucleotide kinase (PNK). This method was also successfully applied in the screening of PNK inhibitors and analysis of the endogenous PNK activity at the single-cell level. We reported a modified exponential amplification reaction strategy and applied it to design an ultrasensitive biosensor for the detection of endogenous polynucleotide kinase activity at single-cell level.
Author Zhang, Yu-Peng
Li, Xiao-Yu
Tang, An-Na
Kong, De-Ming
Du, Yi-Chen
Cui, Yun-Xi
AuthorAffiliation Research Center for Analytical Sciences
Nankai University
State Key Laboratory of Medicinal Chemical Biology
College of Chemistry
Tianjin Key Laboratory of Biosensing and Molecular Recognition
Collaborative Innovation Center of Chemical Science and Engineering (Tianjin)
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BackLink https://www.ncbi.nlm.nih.gov/pubmed/31192323$$D View this record in MEDLINE/PubMed
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Snippet We applied a modified exponential amplification reaction (EXPAR) strategy to design a label-free and "one-pot" biosensor for ultrasensitive detection of...
We applied a modified exponential amplification reaction (EXPAR) strategy to design a label-free and “one-pot” biosensor for ultrasensitive detection of...
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SubjectTerms Amplification
Biosensors
chemical compounds
chemical reactions
Design modifications
Kinases
screening
Signal to noise ratio
Title A modified exponential amplification reaction (EXPAR) with an improved signal-to-noise ratio for ultrasensitive detection of polynucleotide kinase
URI https://www.ncbi.nlm.nih.gov/pubmed/31192323
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https://www.proquest.com/docview/2248372922
https://www.proquest.com/docview/2271823256
Volume 55
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