A modified exponential amplification reaction (EXPAR) with an improved signal-to-noise ratio for ultrasensitive detection of polynucleotide kinase

We applied a modified exponential amplification reaction (EXPAR) strategy to design a label-free and "one-pot" biosensor for ultrasensitive detection of polynucleotide kinase (PNK). This method was also successfully applied in the screening of PNK inhibitors and analysis of the endogenous...

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Published inChemical communications (Cambridge, England) Vol. 55; no. 53; pp. 7611 - 7614
Main Authors Zhang, Yu-Peng, Cui, Yun-Xi, Li, Xiao-Yu, Du, Yi-Chen, Tang, An-Na, Kong, De-Ming
Format Journal Article
LanguageEnglish
Published England Royal Society of Chemistry 07.07.2019
Subjects
Amplification
Biosensors
chemical compounds
chemical reactions
Design modifications
Kinases
screening
Signal to noise ratio
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Summary:We applied a modified exponential amplification reaction (EXPAR) strategy to design a label-free and "one-pot" biosensor for ultrasensitive detection of polynucleotide kinase (PNK). This method was also successfully applied in the screening of PNK inhibitors and analysis of the endogenous PNK activity at the single-cell level. We reported a modified exponential amplification reaction strategy and applied it to design an ultrasensitive biosensor for the detection of endogenous polynucleotide kinase activity at single-cell level.
Bibliography:Electronic supplementary information (ESI) available. See DOI
10.1039/c9cc03568k
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ISSN:1359-7345
1364-548X
1364-548X
DOI:10.1039/c9cc03568k