Specific Removal of C-Reactive Protein by Apheresis in a Porcine Cardiac Infarction Model

• Published in: Blood purification Vol. 31; no. 1-3; pp. 9 - 17
• Main Authors: Slagman, Anna Christine, Bock, Christopher, Abdel-Aty, Hassan, Vogt, Birgit, Gebauer, Frank, Janelt, Gunnar, Wohlgemuth, Franziska, Morgenstern, Rene, Yapici, Gülcan, Puppe, Astrid, Modersohn, Diethelm, Mans, Dörte, Jerichow, Timo, Ott, Sascha, Kunze, Rudolf, Schrödl, Wieland, Janko, Christina, Hermann, Martin, Kalden, Joachim R., Kern, Peter, Parsch, Hans, Kirschfink, Michael, Schulz-Menger, Jeanette, Röttgen, Rainer, Unger, Juliane K., Frei, Ulrich, Schindler, Ralf, Möckel, Martin, Sheriff, Ahmed
• Format: Journal Article
• Language: English
• Published: Basel, Switzerland Karger 01.01.2011
• Subjects: Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Animals; Biological and medical sciences; Blood Component Removal - methods; Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis; C-Reactive Protein - isolation & purification; Female; Medical sciences; Myocardial Infarction - blood; Myocardial Infarction - pathology; Myocardial Infarction - therapy; Original Paper; Swine; Transfusions. Complications. Transfusion reactions. Cell and gene therapy; Acute phase protein; C-reactive protein; Acute myocardial infarction; Apheresis; Adsorber; Vertebrata; Mammalia; Exeresis; Artiodactyla; C reactive protein; Ungulata; Pig
• ISSN: 0253-5068; 1421-9735
• DOI: 10.1159/000320763

Background: C-reactive protein (CRP) is a possible causative factor of the destructive processes observed during the weeks after myocardial infarction. Methods: We developed a clinically relevant animal model including the removal of CRP from blood plasma utilizing a specific CRP adsorber and the visualization of the infarct scar in the living animal by cardiovascular magnetic resonance imaging as a tool to investigate the impact of CRP after acute myocardial infarction. Results: We describe the facets of this model system and kinetics of clinical blood parameters like CRP and troponin. In addition, we demonstrate the potency of CRP apheresis reducing CRP levels by ∼70% in the established treatment system. Conclusion: We showed for the first time that it is possible to conduct apheresis at the following 2 days after acute myocardial infarction in a porcine infarction model and to analyze the infarct by cardiovascular magnetic resonance imaging at day 1 and 14.