Microsatellite markers isolated from polyploid Kielmeyera coriacea Mart. & Zucc. (Clusiaceae) from an enriched genomic library

• Published in: Conservation genetics Vol. 10; no. 5; pp. 1533 - 1535
• Main Authors: Caddah, Mayara K, Campos, Tatiana, Sforça, Danilo A, de Sousa, Adna C. B, do Carmo E. do Amaral, Maria, Bittrich, Volker, de Souza, Anete P
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Springer Netherlands 01.10.2009; Springer Netherlands; Springer Nature B.V
• Subjects: Animal Genetics and Genomics; Anthropogenic factors; Biodiversity; Biomedical and Life Sciences; cerrado; Clusiaceae; Conservation Biology/Ecology; Ecology; Evolutionary Biology; Genetic diversity; Genetic markers; genetic variation; genomic libraries; Hybridization; Kielmeyera; Life Sciences; loci; microsatellite repeats; Plant Genetics and Genomics; polymerase chain reaction; Polymorphism; Polyploidy; Technical Note; Trees; Molecular markers; SSR; Cerrado; Kielmeyera
• ISSN: 1566-0621; 1572-9737
• DOI: 10.1007/s10592-008-9786-8

Kielmeyera coriacea is a morphologically highly variable polyploid species, characteristic of the Cerrado, the savanna-like vegetation of the Central-Brazil. It was subject of various biological studies and, like others cerrado plants, this species suffers strong anthropogenic pressure. To access the genetic diversity of populations of this species, its taxonomic circumscriptions and to study supposed hybridization events, we have developed 11 polymorphic microsatellite loci from a CT/GT-enriched genomic library. The band pattern showed 12-32 bands per locus, and 2-8 bands per locus per individual. All microsatellites successfully amplified PCR products in K. grandiflora, the putative sister species, and all revealed similar multibanded pattern.