Microwave Monitoring of Single Cell Monocytes Subjected to Electroporation

This paper presents the monitoring of single cells subjected to electroporation using microwave dielectric spectroscopy. The experimental results showed first a clear distinction between two cell states: viable cells and affected ones by a chemical treatment (Saponin). It also pointed out a high cor...

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Bibliographic Details
Published inIEEE transactions on microwave theory and techniques Vol. 65; no. 9; pp. 3512 - 3518
Main Authors Tamra, Amar, Dubuc, David, Rols, Marie-Pierre, Grenier, Katia
Format Journal Article
LanguageEnglish
Published New York IEEE 01.09.2017
The Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Institute of Electrical and Electronics Engineers
Subjects
Biomembranes
Biosensor
Chemical treatment
Computer architecture
Damage
dielectric spectroscopy
Dielectrics
Electromagnetism
Electroporation
Engineering Sciences
Microfluidics
Microprocessors
microwave analysis
Microwave FET integrated circuits
Microwave integrated circuits
Microwave theory and techniques
Monitoring
Organic chemistry
single cell
Spectroscopy
Spectrum analysis
single cell
electroporation
microwave analysis
dielectric spectroscopy
Biosensor
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Summary:This paper presents the monitoring of single cells subjected to electroporation using microwave dielectric spectroscopy. The experimental results showed first a clear distinction between two cell states: viable cells and affected ones by a chemical treatment (Saponin). It also pointed out a high correlation (R 2 > 0.94) with biological standard techniques in detecting the two types of electroporation: the reversible and irreversible ones. The developed microfluidic and microwave-based sensor exposes a decrease in the capacitive and conductive contrasts of the investigated single cells treated by irreversible electroporation indicating damages at the cellular level, while cells under reversible electroporation present a similar dielectric response to that of the nontreated cells. This result corresponds to results frequently employed in biological studies. More interestingly, a study of the kinetics of the cell's damage induction over time, by electroporation, has been experimentally done, which makes microwave dielectric spectroscopy an attractive technique for cell's electroporation researches.
ISSN:0018-9480
1557-9670
DOI:10.1109/TMTT.2017.2653776