Microwave Monitoring of Single Cell Monocytes Subjected to Electroporation
This paper presents the monitoring of single cells subjected to electroporation using microwave dielectric spectroscopy. The experimental results showed first a clear distinction between two cell states: viable cells and affected ones by a chemical treatment (Saponin). It also pointed out a high cor...
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Published in | IEEE transactions on microwave theory and techniques Vol. 65; no. 9; pp. 3512 - 3518 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
New York
IEEE
01.09.2017
The Institute of Electrical and Electronics Engineers, Inc. (IEEE) Institute of Electrical and Electronics Engineers |
Subjects | |
Online Access | Get full text |
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Summary: | This paper presents the monitoring of single cells subjected to electroporation using microwave dielectric spectroscopy. The experimental results showed first a clear distinction between two cell states: viable cells and affected ones by a chemical treatment (Saponin). It also pointed out a high correlation (R 2 > 0.94) with biological standard techniques in detecting the two types of electroporation: the reversible and irreversible ones. The developed microfluidic and microwave-based sensor exposes a decrease in the capacitive and conductive contrasts of the investigated single cells treated by irreversible electroporation indicating damages at the cellular level, while cells under reversible electroporation present a similar dielectric response to that of the nontreated cells. This result corresponds to results frequently employed in biological studies. More interestingly, a study of the kinetics of the cell's damage induction over time, by electroporation, has been experimentally done, which makes microwave dielectric spectroscopy an attractive technique for cell's electroporation researches. |
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ISSN: | 0018-9480 1557-9670 |
DOI: | 10.1109/TMTT.2017.2653776 |