Contamination of Bone Marrow Products with an Actinomycete Resembling Microbacterium Species and Reinfusion into Autologous Stem Cell and Bone Marrow Transplant Recipients

• Published in: Clinical infectious diseases Vol. 36; no. 10; pp. e115 - e121
• Main Authors: Hirji, Zahir, Saragosa, Ronnie, Dedier, Heln, Crump, Michael, Franke, Norman, Burrows, Lori, Jamieson, Frances, Brown, Shirley, Gardam, Michael A.
• Format: Journal Article
• Language: English
• Published: United States The University of Chicago Press 15.05.2003
• Subjects: Actinobacteria - classification; Actinobacteria - genetics; Actinobacteria - isolation & purification; Bone Marrow - microbiology; Bone Marrow Transplantation - adverse effects; Cryopreservation; DNA Fingerprinting; Humans; Stem Cells - microbiology; Transplantation, Autologous - adverse effects
• ISSN: 1058-4838; 1537-6591
• DOI: 10.1086/374051

Bacterial contamination of bone marrow or peripheral blood stem cell transplant products typically occurs with skin flora or, rarely, gram-negative organisms. We describe a clonal outbreak of contamination in transplant products caused by contamination with an aerobic actinomycete that occurred at our institution during the summer of 2001. From 1 July through 12 September 2001, 73 peripheral blood or bone marrow stem cell products were obtained from 39 patients, and 34 products were found to be contaminated with the outbreak strain. Fourteen patients were reinfused with contaminated cells, and the outbreak strain was isolated from the blood cultures for one patient. Investigation revealed multiple potential sources for contamination during the product cryopreservation process. The outbreak of contamination was aborted upon modification of the cryopreservation process.