RASGRP1 targeted by H3K27me3 regulates myoblast proliferation and differentiation in mice and pigs

• Published in: Acta biochimica et biophysica Sinica Vol. 56; no. 3; pp. 452 - 461
• Main Authors: Xiao, Liyao, Qiao, Jiaxin, Huang, Yiyang, Tan, Baohua, Hong, Linjun, Li, Zicong, Cai, Gengyuan, Wu, Zhenfang, Zheng, Enqin, Wang, Shanshan, Gu, Ting
• Format: Journal Article
• Language: English
• Published: China Science Press 28.02.2024; China Science Publishing & Media Ltd
• Subjects: Animals; Cell Differentiation - genetics; cell proliferation; Cell Proliferation - genetics; Guanine Nucleotide Exchange Factors - genetics; Guanine Nucleotide Exchange Factors - metabolism; H3K27me3; Histones - metabolism; Mice; Muscle, Skeletal - metabolism; myoblasts; Myoblasts - metabolism; RASGRP1; skeletal muscle; Swine; myoblasts; cell proliferation; skeletal muscle; RASGRP1; H3K27me3
• ISSN: 1672-9145; 1745-7270
• DOI: 10.3724/abbs.2024011

Skeletal muscle is not only the largest organ in the body that is responsible for locomotion and exercise but also crucial for maintaining the body's energy metabolism and endocrine secretion. The trimethylation of histone H3 lysine 27 (H3K27me3) is one of the most important histone modifications that participates in muscle development regulation by repressing the transcription of genes. Previous studies indicate that the gene is regulated by H3K27me3 in embryonic muscle development in pigs, but its function and regulatory role in myogenesis are still unclear. In this study, we verify the crucial role of H3K27me3 in regulation. The gain/loss function of in myogenesis regulation is performed using mouse myoblast C2C12 cells and primarily isolated porcine skeletal muscle satellite cells (PSCs). The results of qPCR, western blot analysis, EdU staining, CCK-8 assay and immunofluorescence staining show that overexpression of promotes cell proliferation and differentiation in both skeletal muscle cell models, while knockdown of leads to the opposite results. These findings indicate that plays an important regulatory role in myogenesis in both mice and pigs.