Lack of Alpha-Synuclein Modulates Microglial Phenotype In Vitro
Alpha (α)-synuclein neuronal effects are continually being defined although its role in regulating glial phenotypes remains unclear. An ability to regulate microglial activation was investigated using primary cultures from wild type and α-synuclein deficient mice ( Snca − / − ). Snca − / − microglia...
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Published in | Neurochemical research Vol. 36; no. 6; pp. 994 - 1004 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Boston
Springer US
01.06.2011
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Alpha (α)-synuclein neuronal effects are continually being defined although its role in regulating glial phenotypes remains unclear. An ability to regulate microglial activation was investigated using primary cultures from wild type and α-synuclein deficient mice (
Snca
−
/
−
).
Snca
−
/
−
microglia demonstrated increased secretion of the cytokine tumor necrosis factor-alpha (TNF-α), impaired phagocytic ability, elevated prostaglandin levels, and increased protein levels of key enzymes in lipid-mediated signaling events, cytosolic phospholipase (cPLA
2
), cyclooxygenase-2 (Cox-2) and phospholipase D2 (PLD2) when compared to wild type cells. Increased cytokine secretion and cPLA
2
and Cox-2 levels in
Snca
−
/
−
microglia were partially attenuated by inhibiting PLD-dependent signaling with n-butanol treatment. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0364-3190 1573-6903 |
DOI: | 10.1007/s11064-011-0439-9 |