Synchronization of Plasmodium falciparum erythrocytic stages in culture

Synchronous development of the erythrocytic stages of a human malaria parasite, Plasmodium falciparum, in culture was accomplished by suspending cultured parasites in 5% D-sorbitol and subsequent reintroduction into culture. Immediately after sorbitol treatment, cultures consisted mainly of single a...

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Bibliographic Details
Published inThe Journal of parasitology Vol. 65; no. 3; p. 418
Main Authors Lambros, C, Vanderberg, J P
Format Journal Article
LanguageEnglish
Published United States 01.06.1979
Subjects
Animals
Cells, Cultured
Culture Media
Erythrocytes - parasitology
Humans
Plasmodium falciparum - growth & development
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Summary:Synchronous development of the erythrocytic stages of a human malaria parasite, Plasmodium falciparum, in culture was accomplished by suspending cultured parasites in 5% D-sorbitol and subsequent reintroduction into culture. Immediately after sorbitol treatment, cultures consisted mainly of single and multiple ring-form infections. At the same time, varying degrees of lysis of erythrocytes infected with the more mature stages of the parasite was evident. Approximately 95% of the parasites were in the ring stage of development at 48 and 96 hr after sorbitol treatment-likewise, a high percentage of trophozoite and schizont stages was observed at 24, 72, and 120 hr. D-Mannitol produced similar, selective, lytic effects.
ISSN:0022-3395
DOI:10.2307/3280287