Bcl-2 Overexpression Abolishes Early Calcium Waving Preceding Apoptosis in NIH-3T3 Murine Fibroblasts

Overexpression of the apoptosis protection gene bcl-2 abolished the earliest response to serum withdrawal in NIH-3T3 murine fibroblasts, i.e., the abrupt cytoplasmic free calcium drop. This phenomenon, also observed in a myeloid cell line, led us to propose this ionic waving as an early apoptotic si...

Full description

Saved in:
Bibliographic Details
Published inBiochemical and biophysical research communications Vol. 204; no. 1; pp. 84 - 90
Main Authors Magnelli, L., Cinelli, M., Turchetti, A., Chiarugi, V.P.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 14.10.1994
Subjects
3T3 Cells
Animals
Apoptosis - physiology
Calcium - metabolism
Culture Media, Serum-Free
Fibroblasts - cytology
Fibroblasts - metabolism
Fibroblasts - physiology
Gene Expression
GTP-Binding Proteins - biosynthesis
Humans
Kinetics
Mice
Proto-Oncogene Proteins - biosynthesis
Proto-Oncogene Proteins c-bcl-2
Time Factors
Transfection
Online AccessGet full text

Cover

More Information
Summary:Overexpression of the apoptosis protection gene bcl-2 abolished the earliest response to serum withdrawal in NIH-3T3 murine fibroblasts, i.e., the abrupt cytoplasmic free calcium drop. This phenomenon, also observed in a myeloid cell line, led us to propose this ionic waving as an early apoptotic signal. Its abolition by bcl-2 overexpression suggests that this gene plays a role also on early events "priming" apoptosis.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
ObjectType-Feature-2
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1994.2429