Systemic and intestinal antibody secreting cell responses and protection in gnotobiotic pigs immunized orally with attenuated Wa human rotavirus and Wa 2/6-rotavirus-like-particles associated with immunostimulating complexes

• Published in: Vaccine Vol. 20; no. 13; pp. 1741 - 1753
• Main Authors: Iosef, Cristiana, Van Nguyen, Trang, Jeong, Kwang-il, Bengtsson, Karin, Morein, Bror, Kim, Yunjeong, Chang, Kyeong-Ok, Azevedo, Marli S.P, Yuan, Lijuan, Nielsen, Paul, Saif, Linda J
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 15.03.2002; Elsevier
• Subjects: Administration, Oral; Animals; Antibodies, Viral - biosynthesis; Antibody-Producing Cells - immunology; Biological and medical sciences; Epidemiology. Vaccinations; Fundamental and applied biological sciences. Psychology; General aspects; Germ-Free Life; Human rotavirus; Humans; Immunoglobulin A - biosynthesis; Immunoglobulin G - biosynthesis; Immunostimulating-complexes (ISCOMs); Infectious diseases; Intestines - immunology; ISCOMs - administration & dosage; Lymphoid Tissue - immunology; Medical sciences; Microbiology; Rotavirus; Rotavirus - immunology; Rotavirus - pathogenicity; Swine; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies; Vaccines, Synthetic - administration & dosage; Viral Vaccines - administration & dosage; Virology; Virus-like-particle (VLP) vaccine; Immunostimulating-complexes (ISCOMs); Rotavirus; Virus-like-particle (VLP) vaccine; Immunization; Human rotavirus; Digestive system; Antibody; Gut; Reoviridae; Pig; Virus; Vertebrata; Mammalia; Artiodactyla; Ungulata
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/S0264-410X(02)00031-2

The undesirable side effects and variable efficacy of some oral live rotavirus vaccines in infants have necessitated alternative vaccine approaches. We evaluated a recombinant RFVP2/WaVP6 rotavirus-like-particle (2/6VLP) oral vaccine, using an immunostimulating complex (ISCOM) matrix as adjuvant, in a gnotobiotic (Gn) pig model of human rotavirus (HRV) disease. The 2/6VLPs adhered to the ISCOM-matrix (2/6VLP-ISCOM ) and were antigenic, but they failed to induce protection. However, when combined with attenuated (Att) HRV for oral priming, the 2/6VLP-ISCOM vaccine was effective as a booster and induced partial protection against virulent Wa HRV. The 250 μg 2/6VLP dose was more effective than 100 μg. The highest mean numbers of IgA antibody secreting cells evaluated by ELISPOT in intestinal lymphoid tissues were in pigs receiving AttHRV+2/6VLP-ISCOM or three doses of AttHRV and were associated with the highest protection rates.