Complete genome sequence of a previously undescribed monopartite begomovirus and betasatellite infecting Malvastrum coromandelianum in Cambodia

• Published in: Archives of virology Vol. 166; no. 6; pp. 1789 - 1793
• Main Authors: Tang, Yafei, Li, Zhenggang, She, Xiaoman, Yu, Lin, Lan, Guobing, Lv, Lihua, Brown, Judith K., He, Zifu
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01.06.2021; Springer Nature B.V
• Subjects: Annotated Sequence Record; Begomovirus; Biomedical and Life Sciences; Biomedicine; Circular DNA; Deoxyribonucleic acid; DNA; Genomes; Infectious Diseases; Malvastrum coromandelianum; Medical Microbiology; Nucleotide sequence; Plant virus diseases; Virology; Viruses; Cambodia
• ISSN: 0304-8608; 1432-8798
• DOI: 10.1007/s00705-021-05016-y

A previously undescribed monopartite begomovirus was identified in Kampot province, Cambodia, in Malvastrum coromandelianum plants exhibiting yellow vein symptoms characteristic of begomovirus infections. The apparently full-length viral component was cloned and sequenced following enrichment of circular DNA by rolling-circle amplification and restriction enzyme digestion. The genome of the virus was 2737 nucleotides in length (KP188831) and exhibited an organization like that of other monopartite begomoviruses, sharing the highest nucleotide sequence similarity (87.7% identity) with ageratum yellow vein virus (AM940137). A satellite molecule was amplified from total DNA by PCR amplification, using the betasatellite-specific primer pair β01/β02. The satellite molecule (1346 nt, KP188832) had structural characteristics like those of other betasatellites associated with begomoviruses and shared the highest nucleotide sequence similarity (84.8% identity) with malvastrum yellow vein betasatellite (MN205547). According to the criteria established for species demarcation for classification of begomoviruses (family Geminiviridae ) and betasatellites (family Tolecusatellitidae ), respectively, the virus isolate from M. coromandelianum in Cambodia is a previously undescribed novel monopartite begomovirus, for which the name “malvastrum yellow vein Cambodia virus” (MaYVCV) is proposed, and the betasatellite is a previously undescribed novel betasatellite, for which the name “malvastrum yellow vein Cambodia betasatellite” (MaYVKHB) is proposed.