Trifunctional integrated DNA-based universal sensing platform for detection of diverse biomolecules in one-pot isothermal exponential amplification mode

A biosensor with all the advantages of ultra-high sensitivity, easy operation, straightforward signal output and universal applicability is introduced. The biosensor was demonstrated to work well in the detection of polynucleotide kinase and DAM methyltransferase, thus providing a powerful tool for...

Full description

Saved in:
Bibliographic Details
Published inChemical communications (Cambridge, England) Vol. 55; no. 53; pp. 763 - 766
Main Authors Cui, Yun-Xi, Feng, Xue-Nan, Li, Xiao-Yu, Zhang, Yu-Peng, Tang, An-Na, Kong, De-Ming
Format Journal Article
LanguageEnglish
Published England Royal Society of Chemistry 27.06.2019
Subjects
Biomolecules
Biosensors
chemical compounds
chemical reactions
drugs
Kinases
methyltransferases
screening
therapeutics
Online AccessGet full text

Cover

More Information
Summary:A biosensor with all the advantages of ultra-high sensitivity, easy operation, straightforward signal output and universal applicability is introduced. The biosensor was demonstrated to work well in the detection of polynucleotide kinase and DAM methyltransferase, thus providing a powerful tool for clinical diagnosis, drug screening and disease therapeutic assay. Template constructed with only a short single-stranded DNA is necessary; it is first recognized by the target enzyme, then multiple amplification cycles are initiated, achieving ultra-high sensitivity by a one-pot isothermal reaction.
Bibliography:Electronic supplementary information (ESI) available: Experimental section and supplementary figures S1-S15. See DOI
10.1039/c9cc03758f
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
ISSN:1359-7345
1364-548X
1364-548X
DOI:10.1039/c9cc03758f