Construction and Serological Characterization of a Recombinant Human Single Chain T-Cell Receptor

• Published in: Biochemical and biophysical research communications Vol. 201; no. 3; pp. 1502 - 1509
• Main Authors: Lake, D.F., Bernstein, R.M., Hersh, E.M., Kaymaz, H., Schluter, S.F., Marchalonis, J.J.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 30.06.1994
• Subjects: Autoantibodies - immunology; Base Sequence; DNA Primers - chemistry; Humans; In Vitro Techniques; Molecular Sequence Data; Protein Structure, Tertiary; Receptors, Antigen, T-Cell, alpha-beta - chemistry; Receptors, Antigen, T-Cell, alpha-beta - genetics; Receptors, Antigen, T-Cell, alpha-beta - immunology; Recombinant Proteins - immunology; Tumor Cells, Cultured
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1006/bbrc.1994.1874

A single chain T cell receptor (scTcr) was constructed from the complete Vα and Vβ regions of Jurkat T-cell receptor α/β chain genes using molecular cloning techniques. The recombinant scTcr reacted with a panel of rabbit antisera generated against synthetic 16-mer peptides duplicating the amino acid sequence of Jurkat Vα and β chains but not with antisera directed against peptides from the constant domain. Autoantibodies present in sera from systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA) patients bound the scTcr in ELISA assays. The recombinant scTcr described here should prove to be a useful reagent with which to study T-cell receptor activity in serological and functional assays.