Cytoplasmic transformation: Mitochondria of wild-type Baker's yeast restoring respiratory capacity in the respiratory deficient “petite” mutant
Respiratory deficient cells of S.cerevisiae , which lack the extrachromosomal genetic determinant of respiratory capacity, were converted into their spheroplasts and the latter were treated with mitochondria of a respiring yeast strain. A sizeable portion of the treated cells acquired the ability to...
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| Published in | Biochemical and biophysical research communications Vol. 20; no. 6; pp. 733 - 738 |
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| Main Authors | , |
| Format | Journal Article |
| Language | English |
| Published |
Elsevier Inc
01.09.1965
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| Online Access | Get full text |
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| Summary: | Respiratory deficient cells of
S.cerevisiae
, which lack the extrachromosomal genetic determinant of respiratory capacity, were converted into their spheroplasts and the latter were treated with mitochondria of a respiring yeast strain. A sizeable portion of the treated cells acquired the ability to respire and to form normal-sized colonies, indicating that mitochondria had been incorporated into them. Mitochondria may thus be regarded as transmissible carriers of cytoplasmic genes. |
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| ISSN: | 0006-291X 1090-2104 |
| DOI: | 10.1016/0006-291X(65)90078-1 |