Pepsinogen D. A fourth proteolytic zymogen from pig gastric mucosa

Methods are described for the isolation and purification of pepsinogen D, a minor zymogen occurring to the extent of about 5% of the potential proteolytic activity in neutral extracts of the pig gastric mucosa. The physical and chemical properties of this zymogen indicate that it is very similar to,...

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Bibliographic Details
Published inBiochemical journal Vol. 104; no. 3; pp. 735 - 741
Main Authors Lee, D, Ryle, A P
Format Journal Article
LanguageEnglish
Published England 01.09.1967
Subjects
Amino Acid Sequence
Animals
Cattle
Chromatography
Chymotrypsin - metabolism
Dialysis
Electrophoresis
Enzyme Precursors - analysis
Gastric Mucosa - enzymology
Hemoglobins - metabolism
Milk - drug effects
Pepsin A
Peptide Hydrolases - metabolism
Staining and Labeling
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Summary:Methods are described for the isolation and purification of pepsinogen D, a minor zymogen occurring to the extent of about 5% of the potential proteolytic activity in neutral extracts of the pig gastric mucosa. The physical and chemical properties of this zymogen indicate that it is very similar to, if not identical with, dephosphopepsinogen.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Present address: Department of Biochemistry, University of Manitoba, Medical Buildings, Bannatyne and Emily, Winnipeg, 3, Canada.
ISSN:0264-6021
1470-8728
DOI:10.1042/bj1040735