Differentially expressed proteins associated with myogenesis and adipogenesis in skeletal muscle and adipose tissue between bulls and steers

• Published in: Molecular biology reports Vol. 39; no. 2; pp. 953 - 960
• Main Authors: Zhang, Qiankun, Lee, Hong-Gu, Han, Jung-A, Kang, Sang Kee, Lee, Nam Kyung, Baik, Myunggi, Choi, Yun-Jaie
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.02.2012; Springer Nature B.V
• Subjects: Adipogenesis - physiology; Adipose Tissue - metabolism; Animal Anatomy; Animal Biochemistry; Animals; Ankyrin Repeat - genetics; Biomedical and Life Sciences; Body fat; Castration; Cattle; Cofilin 2 - metabolism; Electrophoresis, Gel, Two-Dimensional; Fluorescent Antibody Technique; Gene Expression Regulation - genetics; Gene Expression Regulation - physiology; Histology; HSP27 Heat-Shock Proteins - metabolism; Image Processing, Computer-Assisted; L-Lactate Dehydrogenase - metabolism; Life Sciences; Male; Molecular biology; Morphology; Muscle Development - physiology; Muscle, Skeletal - metabolism; Myosin Light Chains - metabolism; Orchiectomy; Proteins; Proteins - metabolism; Proteomics; Real-Time Polymerase Chain Reaction; Ribonucleic acid; RNA; Testosterone - deficiency; Transaldolase - metabolism; Bovine embryonic fibroblasts; Castration; Myogenesis; Proteomics; Adipogenesis
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-011-0821-3

The objective of this study was to identify some proteins associated with testosterone-related differences in myogenesis and adipogenesis between bulls and steers. Global proteins were monitored in skeletal muscle and adipose tissue from bulls ( n  = 20) and steers ( n  = 20), respectively. We identified four differentially expressed (twofold or more) proteins in skeletal muscle from bulls, myosin light chain 1 (MLC1), ankyrin repeat domain-containing protein 1 (ANKRD1) and heat shock protein beta 1 (HSPB1) that were up-regulated and cofilin 2 (CFL2) that was down-regulated, and also identified two down-regulated proteins in adipose tissue, transaldolase 1 (TALDO1) and l -lactate dehydrogenase B chain (LDHB). In vitro, after myogenic differentiation of a bovine cell line, the mRNA expression of HSPB1 not only increased approximately tenfold in response to differentiation but threefold in response to testosterone addition, respectively, but that of ANKRD1 and CFL2 did not significantly change in response to myogenic differentiation or testosterone addition. Likewise, after adipogenic differentiation of a bovine cell line, the mRNA expression of TALDO1 and LDHB did not significantly vary in response to adipogenic differentiation or testosterone addition. Therefore, we suggest that HSPB1 could have an important role during testosterone-related myogenesis.