The comparision among euploidy of preimplantation blastocysts in different controlled ovary stimulation (COH) protocols

• Published in: Archives of gynecology and obstetrics Vol. 310; no. 3; pp. 1687 - 1695
• Main Authors: Wang, Yu, Xu, Junting, Yin, Xiangjie, Fang, Yuan, Li, Kunming
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.09.2024; Springer Nature B.V
• Subjects: Adult; Aneuploidy; Blastocyst; Embryos; Endocrinology; Female; Gonadotropin-Releasing Hormone - agonists; Gonadotropin-Releasing Hormone - antagonists & inhibitors; Gynecologic Endocrinology and Reproductive Medicine; Gynecology; Human Genetics; Humans; Medicine; Medicine & Public Health; Obstetrics/Perinatology/Midwifery; Ovaries; Ovulation Induction - methods; Pregnancy; Preimplantation Diagnosis; Protocol; Retrospective Studies; Sperm Injections, Intracytoplasmic; Ovarian stimulation; Embryo euploidy rate per oocyte retrieved; Preimplantation genetic testing for aneuploidy; Cycle cancellation rate; Euploidy rate per embryo biopsied
• ISSN: 1432-0711; 0932-0067; 1432-0711
• DOI: 10.1007/s00404-024-07474-6

Purpose To compare differences in euploidy rates for blastocysts in preimplantation genetic testing for aneuploidy (PGT-A) cycles after gonadotropin-releasing hormone agonist (GnRH-a) long and short protocols, GnRH-antagonist (GnRH-ant) protocol, progestin-primed ovarian stimulation and mild stimulation protocols, and other ovary stimulation protocols. Methods This was a retrospective cohort study from the Assisted Reproductive Medicine Department of Shanghai First Maternity and Infant Hospital. A total of 1657 PGT-A cycles with intracytoplasmic sperm injection after different controlled ovary hyperstimulation protocols were analyzed, and a total of 3154 embryos were biopsied. Differences in euploidy rate per embryo biopsied, embryo euploidy rate per oocyte retrieved and cycle cancellation rate were compared. Results For the PGT-A cycles, the euploidy rate per embryo biopsied was lower in the GnRH-ant protocol than in the GnRH-a long protocol (53.26 vs. 58.68%, respectively). Multiple linear regression showed that the GnRH-ant protocol was associated with a lower euploidy rate per embryo biopsied ( β  =  −0.079, p  = 0.011). The euploidy rate per embryo biopsied was not affected by total gonadotropin dosage, duration of stimulation and number of oocytes retrieved. The embryo euploidy rate per oocyte retrieved was similar in all protocols and was negatively correlated with the total number of oocytes retrieved ( β  =  −0.003, p  = 0.003). Conclusion Compared with the GnRH-a long protocol, the GnRH-ant protocol was associated with a lower euploidy rate per embryo biopsied. The total gonadotropin dosage, duration of stimulation and number of oocytes retrieved did not appear to significantly influence euploidy rates.