Enhanced Performance of Immobilized Rhizopus oryzae Lipase on Coated Porous Polypropylene Support with Additives

The immobilization of Rhizopus oryzae lipase (RoL) by hydrophobic adsorption on polypropylene supports with additives was investigated. Additives such as hen egg albumin, sodium caseinate and CAVAMAX® W6 were used to coat the support during immobilization where the immobilized RoL on coated support...

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Bibliographic Details
Published inCatalysts Vol. 11; no. 3; p. 303
Main Authors Sani, Fatimah, Mokhtar, Nur Fathiah, Mohamad Ali, Mohd Shukuri, Raja Abd Rahman, Raja Noor Zaliha
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 2021
Subjects
additive
Additives
Adsorption
Albumins
Catalysts
Catalytic activity
Chemical reactions
Eggs
Enzymes
Esterification
Fungi
Immobilization
Lipase
Morphology
Optimization
Performance enhancement
Polypropylene
Rhizopus oryzae
Sodium
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Summary:The immobilization of Rhizopus oryzae lipase (RoL) by hydrophobic adsorption on polypropylene supports with additives was investigated. Additives such as hen egg albumin, sodium caseinate and CAVAMAX® W6 were used to coat the support during immobilization where the immobilized RoL on coated support was compared to those of noncoated support. Following the immobilization, the catalytic activity of immobilized RoL was characterized based on different temperatures and pH. The immobilized RoL without additives showed optimal lipase activity at an optimum temperature of 50 °C and pH 6. However, RoL lipase that was immobilized on support treated with CAVAMAX® W6 had better performance in terms of hydrolytic activity and stability as compared to other additives. In addition, by having a support treated with hen egg albumin, the immobilized RoL was capable of yielding higher ester during esterification reactions.
ISSN:2073-4344
2073-4344
DOI:10.3390/catal11030303