Radioimmunoassay of colchicine

Antibodies to cholchicine were raised in rabbits after injection of N-desacetylthiocolchicine conjugated to bovine serum albumin. Antisera diluted 1:100 bound 50% of 1.6ng of [3H]colchicine. Cross-reaction of the antisera with colchicine derivatives and other drugs was measured and a radioimmunoassa...

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Bibliographic Details
Published inBiochemical journal Vol. 151; no. 2; pp. 413 - 415
Main Authors Boudene, C, Duprey, F, Bohuon, C
Format Journal Article
LanguageEnglish
Published England 01.11.1975
Subjects
Animals
Antibody Specificity
Chromatography, Gel
Colchicine - analysis
Colchicine - immunology
Haptens
Rabbits - immunology
Radioimmunoassay
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Summary:Antibodies to cholchicine were raised in rabbits after injection of N-desacetylthiocolchicine conjugated to bovine serum albumin. Antisera diluted 1:100 bound 50% of 1.6ng of [3H]colchicine. Cross-reaction of the antisera with colchicine derivatives and other drugs was measured and a radioimmunoassay of colchicine in biological fluids was proposed.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0264-6021
1470-8728
DOI:10.1042/bj1510413