Chick Liver-Storage Bioassay of Alpha-Tocopherol: METHODS

The results of both a long (two-week supplementation) multiple slope-ratio chick liver-storage bioassay and a short (three-day supplementation, one-day stabilization) bioassay to tocopherol indicated that the different forms of α-tocopherol were utilized to the same relative degree under the two set...

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Bibliographic Details
Published inThe Journal of nutrition Vol. 75; no. 2; pp. 165 - 174
Main Authors Dicks, Martha W., Matterson, L.D.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.10.1961
Subjects
alpha-Tocopherol
Animals
Biological Assay
Chickens
Liver - metabolism
Old Medline
Vitamin E - chemistry
VITAMIN E/chemistry
LIVER/metabolism
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Summary:The results of both a long (two-week supplementation) multiple slope-ratio chick liver-storage bioassay and a short (three-day supplementation, one-day stabilization) bioassay to tocopherol indicated that the different forms of α-tocopherol were utilized to the same relative degree under the two sets of conditions. The d,α-tocopheryl acid succinate in relation to the dl,α-tocopheryl acetate was only 0.61 (short bioassay) to 0.73 and 0.77 (long bioassay) as well utilized by the chick. The method of Bro-Rasmussen and Hjarde ('57a), when modified as to the saponification procedure, with the same procedure of chromatography through secondary magnesium phosphate, and with a modified method of reading with ferric chloride-dipyridyl, was shown to be a possible substitute for the method of molecular distillation and Florex-chromatography for analysis of liver for α-tocopherol. Limitations of the method of saponification followed by secondary magnesium phosphate-chromatography were reported.
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ISSN:0022-3166
DOI:10.1093/jn/75.2.165