Chick Liver-Storage Bioassay of Alpha-Tocopherol: METHODS
The results of both a long (two-week supplementation) multiple slope-ratio chick liver-storage bioassay and a short (three-day supplementation, one-day stabilization) bioassay to tocopherol indicated that the different forms of α-tocopherol were utilized to the same relative degree under the two set...
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Published in | The Journal of nutrition Vol. 75; no. 2; pp. 165 - 174 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
United States
Elsevier Inc
01.10.1961
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Subjects | |
Online Access | Get full text |
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Summary: | The results of both a long (two-week supplementation) multiple slope-ratio chick liver-storage bioassay and a short (three-day supplementation, one-day stabilization) bioassay to tocopherol indicated that the different forms of α-tocopherol were utilized to the same relative degree under the two sets of conditions. The d,α-tocopheryl acid succinate in relation to the dl,α-tocopheryl acetate was only 0.61 (short bioassay) to 0.73 and 0.77 (long bioassay) as well utilized by the chick.
The method of Bro-Rasmussen and Hjarde ('57a), when modified as to the saponification procedure, with the same procedure of chromatography through secondary magnesium phosphate, and with a modified method of reading with ferric chloride-dipyridyl, was shown to be a possible substitute for the method of molecular distillation and Florex-chromatography for analysis of liver for α-tocopherol. Limitations of the method of saponification followed by secondary magnesium phosphate-chromatography were reported. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0022-3166 |
DOI: | 10.1093/jn/75.2.165 |