Biosynthesis of polyketides. Purification and inhibition studies of 6-methylsalicylic acid synthase

• Published in: Bioorganic chemistry Vol. 3; no. 2; pp. 238 - 248
• Main Authors: Scott, A.Ian, Beadling, L.C., Georgopapadakou, N.H., Subbarayan, C.R.
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 01.01.1974
• ISSN: 0045-2068; 1090-2120
• DOI: 10.1016/0045-2068(74)90047-9

6-MSA 3 3 The abbreviations used are: ACP, acyl carrier protein; BSA, bovine serum albumin; β-ME, β-mercaptoethanol; NAC, N-acetylcysteamine; NEM, N-ethylmaleimide; 6-MSA, 6-methylsalicylic acid; TAL, triacetic acid lactone. synthase has been purified 190-fold with 33% yield. The purification was found to be dependent on the presence of glycerol. The acetylenic inhibitors 3-pentynoyl- and 2-hexynoyl-NAC completely inhibit 6-MSA production at concentrations in which fatty acid synthesis, TAL production as well as NADPH oxidation are only partially affected. These results confirm earlier studies on the specificity of inhibition by acetylenic inhibitors and support a mechanism wherein the NADPH-mediated reduction step occurs on a 6-carbon rather than on an 8-carbon intermediate.