Fourier Transform Infrared Spectroscopy and Photoacoustic Spectroscopy for Saliva Analysis

Saliva provides a valuable tool for assessing oral and systemic diseases, but concentrations of salivary components are very small, calling the need for precise analysis methods. In this work, Fourier transform infrared (FT-IR) spectroscopy using transmission and photoacoustic (PA) modes were compar...

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Published inApplied spectroscopy Vol. 70; no. 9; p. 1502
Main Authors Mikkonen, Jopi J W, Raittila, Jussi, Rieppo, Lassi, Lappalainen, Reijo, Kullaa, Arja M, Myllymaa, Sami
Format Journal Article
LanguageEnglish
Published United States 01.09.2016
Subjects
Adult
Albumins - analysis
Female
Humans
Linear Models
Male
Photoacoustic Techniques - methods
Saliva - chemistry
Spectroscopy, Fourier Transform Infrared - methods
Thiocyanates - analysis
Young Adult
PA
oral diseases
photoacoustic
Infrared
spectroscopy
FT-IR
IR
fourier transform infrared spectroscopy
saliva analysis
oral cancer
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Summary:Saliva provides a valuable tool for assessing oral and systemic diseases, but concentrations of salivary components are very small, calling the need for precise analysis methods. In this work, Fourier transform infrared (FT-IR) spectroscopy using transmission and photoacoustic (PA) modes were compared for quantitative analysis of saliva. The performance of these techniques was compared with a calibration series. The linearity of spectrum output was verified by using albumin-thiocyanate (SCN(-)) solution at different SCN(-) concentrations. Saliva samples used as a comparison were obtained from healthy subjects. Saliva droplets of 15 µL were applied on the silicon sample substrate, 6 drops for each specimen, and dried at 37 ℃ overnight. The measurements were carried out using an FT-IR spectrometer in conjunction with an accessory unit for PA measurements. The findings with both transmission and PA modes mirror each other. The major bands presented were 1500-1750 cm(-1) for proteins and 1050-1200 cm(-1) for carbohydrates. In addition, the distinct spectral band at 2050 cm(-1) derives from SCN(-) anions, which is converted by salivary peroxidases to hypothiocyanate (OSCN(-)). The correlation between the spectroscopic data with SCN(-) concentration (r > 0.990 for transmission and r = 0.967 for PA mode) was found to be significant (P < 0.01), thus promising to be utilized in future applications.
ISSN:1943-3530
DOI:10.1177/0003702816654149