Reconstitution of pig lymphocyte plasma membranes from solubilized components, with particular reference to membrane-associated immunoglobulins

Plasma membranes of lymphocytes obtained from pig mesenteric lymph nodes were reconstituted after solubilization with bile salts. The proportion by weight of immunoglobulin in the reconstituted membrane was no greater than about 5-10% of that in the original membrane. Possible reasons for the low re...

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Bibliographic Details
Published inBiochemical journal Vol. 152; no. 2; pp. 267 - 270
Main Authors Chavin, S I, Holliman, A
Format Journal Article
LanguageEnglish
Published England 01.11.1975
Subjects
Animals
Detergents
Electrophoresis, Polyacrylamide Gel
Lymphocytes - ultrastructure
Microscopy, Electron
Receptors, Antigen, B-Cell
Solubility
Subcellular Structures
Swine
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Summary:Plasma membranes of lymphocytes obtained from pig mesenteric lymph nodes were reconstituted after solubilization with bile salts. The proportion by weight of immunoglobulin in the reconstituted membrane was no greater than about 5-10% of that in the original membrane. Possible reasons for the low reincorporation of immunoglobulin are discussed.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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To whom reprint requests should be addressed. Present address: Medical Research Council Laboratory, Oxford Haemophilia Centre, Churchill Hospital, Headington, Oxford OX3 7LJ, U.K.
ISSN:0264-6021
0306-3283
1470-8728
DOI:10.1042/bj1520267