Natural prevalence of entomopathogenic fungi in hibernating pupae of Cameraria ohridella (Lepidoptera: Gracillariidae) and virulence of selected isolates

Spontaneous infection of hibernating Cameraria ohridella pupae by entomopathogenic fungi was monitored at two localities of south-western Slovakia and efficacy of selected fungal isolates against the pupae was evaluated in laboratory. Natural prevalence of fungal infection in pupal populations was l...

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Published inPlant protection science Vol. 52; no. 3; pp. 199 - 208
Main Authors Schemmer, Robert, Chládeková, Petra, Medo, Juraj, Barta, Marek
Format Journal Article
LanguageEnglish
Published Prague Czech Academy of Agricultural Sciences (CAAS) 01.01.2016
Czech Academy of Agricultural Sciences
Subjects
beauveria
Cameraria ohridella
Conidia
Entomopathogenic fungi
Fungi
Growth rate
horse-chestnut leaf miner
Infections
Insects
isaria
metarhizium
Mycelia
Pupae
survival analysis
Virulence
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Summary:Spontaneous infection of hibernating Cameraria ohridella pupae by entomopathogenic fungi was monitored at two localities of south-western Slovakia and efficacy of selected fungal isolates against the pupae was evaluated in laboratory. Natural prevalence of fungal infection in pupal populations was low (< 7%) and varied depending upon collecting date, locality, year, and pathogen. Ten isolates of three fungal species, Beauveria pseudobassiana, Isaria fumosorosea, and I. farinosa, isolated from the naturally infected pupae and three isolates of non-insect origin of two fungi, B. bassiana and Metarhizium anisopliae, were screened for colony growth, conidial production in vitro, and virulence to C. ohridella pupae. A significant variability in the evaluated traits was detected among the isolates. Pupae demonstrated vulnerability to all the isolates irrespective of their origin, however virulence of isolates varied significantly. I. fumosorosea was the most virulent fungus with median lethal concentration of 0.09 × 104 conidia/ml (isolate CO10-IFu) and mean survival time of pupae of 7.14 days (isolate CO8-IFu). No correlation between conidial yield of isolates and fungal virulence was observed, but a moderately strong relationship was detected between virulence and mycelial growth rate of isolates.
ISSN:1212-2580
1805-9341
DOI:10.17221/110/2015-PPS