Mass Spectrometric Sequence Studies of a Superoxide Dismutase from Bacillus stearothermophilus

A partial sequence of the manganese‐containing superoxide dismutase from Bacillus stearothermophilus has been determined by mass spectrometry. A new fragmentation reaction at N‐terminal proline is described and an ‘in‐chain’ fragmentation, also occurring at proline, has been observed. There is some...

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Published inEuropean journal of biochemistry Vol. 113; no. 2; pp. 333 - 338
Main Authors AUFFRET, Anthony D., Blake, Timothy J., WILLIAMS, Dudley H.
Format Journal Article
LanguageEnglish
Published Oxford, UK Blackwell Publishing Ltd 01.01.1981
Subjects
Amino Acid Sequence
Chemical Fractionation
Electrophoresis, Paper
Geobacillus stearothermophilus - enzymology
Mass Spectrometry
Peptide Fragments - isolation & purification
Superoxide Dismutase - analysis
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Summary:A partial sequence of the manganese‐containing superoxide dismutase from Bacillus stearothermophilus has been determined by mass spectrometry. A new fragmentation reaction at N‐terminal proline is described and an ‘in‐chain’ fragmentation, also occurring at proline, has been observed. There is some evidence that the latter cleavage may also occur at other residues. Some differences from the classically determined sequence are reported, and the validity of these observations is discussed.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0014-2956
1432-1033
DOI:10.1111/j.1432-1033.1981.tb05071.x