Dysregulation of epigenetic related genes in Diabetic Trigger finger Patients; preliminary analysis of Patient-Derived Samples

• Published in: Biomolecular concepts Vol. 11; no. 1; pp. 221 - 229
• Main Authors: Cain, Michael, Awad, Mohamed E., Kolhe, Ravindra, Mondal, Ashis K., Ghilzai, Umar, Isales, Carlos, Fulcher, Mark, Fulzele, Sadanand
• Format: Journal Article
• Language: English
• Published: Berlin De Gruyter 31.12.2020; Walter de Gruyter GmbH
• Subjects: Aggrecan; Carpal tunnel syndrome; Diabetes; Diabetes mellitus; Diabetic; DNMT1 protein; Enzymes; Epigenetics; Extracellular matrix; Gene expression; Growth factors; HDAC2 protein; Histone deacetylase; Hyperglycemia; Inflammation; Insulin-like growth factors; Polymerase chain reaction; Trigger Finger
• ISSN: 1868-5021; 1868-5021; 1868-503X
• DOI: 10.1515/bmc-2020-0020

BackgroundTrigger finger (TF), a painful condition involving a finger flexor tendon, is a common problem with a prevalence of ~2-3% in the general population. However, the TF prevalence is higher among diabetic patients-ranges from 6.7% to 10%. We have analyzed the expression of the extracellular matrix, inflammation, and epigenetic related genes in diabetic and non-diabetes TF. We hypothesized that Diabetes condition induces alter the expression of epigenetic modification genes in diabetic patients and one of the underlying determinants for more prevalence of TF in diabetic patients.MethodTissues from the fingers of patients with symptomatic trigger fingers were collected. We had three groups: carpal tunnel syndrome (as a control), trigger finger, and diabetic trigger finger. A quantitative real-time polymerase chain reaction was performed. The gene expression of Extracellular matrix (ECM) components [COL-I, COL-II, COL-X, Aggrecan], DNA methyltransferases enzymes (DNMT1, DNMT3), growth factors (TGF-b, IGF), and Histone deacetylase enzymes (HDAC1, HDAC2) were evaluated in all groups.ResultsThe mRNA expression of COL-I, COL-II, Aggrecan was significantly higher in the pully A1 of diabetic patients (p= 0.0164, p=0.0351, p=0.0399, respectively) as compared to non-diabetic TF patients. Diabetes was associated with a significant increase in the DNMT3 expression compared to non-diabetic TF patients (p=0.0485). HDAC1 and HDAC2 gene expression were up-regulated in diabetic TF than non-diabetic TF.ConclusionThe chronic state of hyperglycemia induces epigenetic modification of gene expressions in trigger fingers. This seems to have a significant impact on the development, recurrence, and progression of trigger finger in diabetic patients.