Transcriptomic and Histological Analysis of the Response of Susceptible and Resistant Cucumber to Meloidogyne incognita Infection Revealing Complex Resistance via Multiple Signaling Pathways
The root-knot nematode (RKN), Meloidogyne incognita , is a devastating pathogen for cucumber ( Cucumis sativus L.) specially in production under protected environments or continuous cropping. High level RKN resistance has been identified in African horned melon Cucumis metuliferus (CM). However, the...
Saved in:
Published in | Frontiers in plant science Vol. 12; p. 675429 |
---|---|
Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Frontiers Media S.A
14.06.2021
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | The root-knot nematode (RKN),
Meloidogyne incognita
, is a devastating pathogen for cucumber (
Cucumis sativus
L.) specially in production under protected environments or continuous cropping. High level RKN resistance has been identified in African horned melon
Cucumis metuliferus
(CM). However, the resistance mechanism remains unclear. In this study, the comparative analysis on phenotypic and transcriptomic responses in the susceptible cucumber inbred line Q24 and the resistant CM, after
M. incognita
infection, was performed. The results showed that, in comparison with Q24, the CM was able to significantly reduce penetration numbers of second stage juveniles (J2), slow its development in the roots resulting in fewer galls and smaller giant cells suggesting the presence of host resistance in CM. Comparative transcriptomes analysis of Q24 and CM before and after
M. incognita
infection was conducted and differentially expressed genes (DEGs) associated with host resistance were identified in CM. Enrichment analyses revealed most enriched DEGs in Ca
2+
signaling, salicylic acid (SA)/jamonate signaling (JA), as well as auxin (IAA) signaling pathways. In particular, in CM, DEGs in the Ca
2+
signaling pathway such as those for the calmodulin and calcium-binding proteins were upregulated at the early stage of
M. incognita
infection; genes for SA/JA synthesis/signal transduction were markedly activated, whereas the IAA signaling pathway genes were inhibited upon infection suggesting the importance of SA/JA signaling pathways in mediating
M. incognita
resistance in CM. A model was established to explain the different molecular mechanisms on
M. incognita
susceptibility in cucumber and resistance to
M. incognita
infection in CM. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Edited by: Claudia S. L. Vicente, University of Évora, Portugal This article was submitted to Plant Pathogen Interactions, a section of the journal Frontiers in Plant Science Reviewed by: Joana M. S. Cardoso, University of Coimbra, Portugal; Tariq Mukhtar, Pir Mehr Ali Shah Arid Agriculture University, Pakistan |
ISSN: | 1664-462X 1664-462X |
DOI: | 10.3389/fpls.2021.675429 |