An electrochemiluminescence assay for gp340 (DMBT1)

• Published in: Analytical biochemistry Vol. 440; no. 1; pp. 78 - 80
• Main Authors: Barber, Cheryl, Weissman, Drew, Barnhart, Kurt, Dalvi, Minal, Abrams, William R., Malamud, Daniel
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.09.2013
• Subjects: antibodies; Cervical vaginal lavage; Electrochemical Techniques - methods; Enzyme-Linked Immunosorbent Assay; Female; Humans; Luminescent Measurements - methods; Male; MesoScale Discovery; Receptors, Cell Surface - analysis; Receptors, Scavenger - analysis; saliva; Saliva - chemistry; Salivary agglutinin; Scavenger receptor cysteine-rich; Sensitivity and Specificity; Vaginal Douching; volunteers; Whole-mouth stimulated saliva; Salivary agglutinin; MesoScale Discovery; Whole-mouth stimulated saliva; Scavenger receptor cysteine-rich; Cervical vaginal lavage
• ISSN: 0003-2697; 1096-0309
• DOI: 10.1016/j.ab.2013.05.015

Gp340 is a member of the scavenger receptor cysteine-rich family of innate immune molecules and also functions as a tumor suppressor. This study describes a picogram-level assay using electrochemiluminescence technology on the MesoScale Discovery platform. Antibodies were evaluated and the best pair was used to assay whole-mouth stimulated saliva and cervical/vaginal lavage. The assay was tested using specimens obtained from healthy volunteers to determine if gp340 concentration in saliva correlates with levels in vaginal lavage fluid. Interestingly, no correlation was determined between gp340 content in these two fluids.