Lymphocytopoiesis, Plasmacytopoiesis and Cellular Proliferation in Nonantigenically Stimulated Germfree Mice

Summary Quantitative evaluation of lymphocytopoiesis and plasmacytopoiesis in various lymph nodes of germfree and conv mice show the conv animals have about 3 times (% basis) or 12 times (absolute basis determined from mesenteric lymph nodes) more blast and potential antibody producing cells than th...

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Bibliographic Details
Published inThe Journal of immunology (1950) Vol. 97; no. 2; pp. 267 - 274
Main Authors Olson, George B, Wostmann, Bernard S
Format Journal Article
LanguageEnglish
Published United States Am Assoc Immnol 01.08.1966
Subjects
Animals
Autoradiography
Germ-Free Life
Hematopoiesis
In Vitro Techniques
Lymph Nodes - cytology
Lymph Nodes - physiology
Lymphocytes
Mice
Plasma Cells
Thymidine - metabolism
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Summary:Summary Quantitative evaluation of lymphocytopoiesis and plasmacytopoiesis in various lymph nodes of germfree and conv mice show the conv animals have about 3 times (% basis) or 12 times (absolute basis determined from mesenteric lymph nodes) more blast and potential antibody producing cells than their germfree counterparts. Other cellular aspects such as cell distribution within respective lymph nodes and proliferation rate of lymphoid cells are not affected by the animal's germfree status. Two hours after tritiated thymidine (TH3) pulse labelling, the nodes of the conv mice contained more labelled cells per 1000 cells than the respective nodes of germfree mice. Evidence suggests this difference was due to a smaller quantity of less differentiated cells and to a restriction of TH3 label movement in nodes of germfree mice. Cells which became labelled incorporated the TH3 to the same extent in both germfree and conv mice.
Bibliography:ObjectType-Article-1
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ISSN:0022-1767
1550-6606
DOI:10.4049/jimmunol.97.2.267