Anti-Aging Activity of Lavandula angustifolia Extract Fermented with Pediococcus pentosaceus DK1 Isolated from Diospyros kaki Fruit in UVB-Irradiated Human Skin Fibroblasts and Analysis of Principal Components

• Published in: Journal of microbiology and biotechnology Vol. 29; no. 1; pp. 21 - 29
• Main Authors: Ha, Ji Hoon, Kim, A Rang, Lee, Keon-Soo, Xuan, Song Hua, Kang, Hee Cheol, Lee, Dong Hwan, Cha, Mi Yeon, Kim, Hye Jin, An, Mi, Park, Soo Nam
• Format: Journal Article
• Language: English
• Published: Korea (South) 한국미생물·생명공학회 01.01.2019
• Subjects: 생물학; UVB; fermentation; procollagen; matrix metalloproteinase-1; Pediococcus pentosaceus DK1
• ISSN: 1017-7825; 1738-8872
• DOI: 10.4014/jmb.1809.09037

extract fermented with DK1 were determined on UVB-mediated MMP-1 expression and collagen decrease in human skin fibroblasts and the conversion of its components. Fermentation was performed at varying extract and MRS medium concentrations, and optimal fermentation conditions were selected. extracts showed decreased cytotoxicity after fermentation in the fibroblasts. UVB-irradiated fibroblasts treated with fermented extract showed MMP-1 expression 8.2-14.0% lower than that in UVB-irradiated fibroblasts treated with non-fermented extract. This was observed even at fermented extract concentrations lower than those of non-fermented extracts. Fibroblasts treated with fermented extract showed 20% less reduction in collagen production upon UVB irradiation than those treated with non-fermented extracts. UVB-irradiated fibroblasts treated with fermented extracts showed 50% higher inhibition of ROS generation than those treated with non-fermented extract. Luteolin and apigenin glycosides of were converted during fermentation, and identified using RP-HPLC and LC/ESI-MS. Therefore, the effects of extract were increased through fermentation by on MMP-1 expression and collagen decrease in UVB-irradiated human skin fibroblasts.